In vitro analysis of UV-damage-induced inhibition of replication.

Effect of Host Src-kinase Inhibition on Replication of Coxsackievirus B3 in vitro

Pomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study

Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus...

CHK1 activity is required for continuous replication fork elongation but not stabilization of post-replicative gaps after UV irradiation

Ultraviolet (UV)-induced DNA damage causes an efficient block of elongating replication forks. The checkpoint kinase, CHK1 has been shown to stabilize replication forks following hydroxyurea treatment. Therefore, we wanted to test if the increased UV sensitivity caused by the unspecific kinase inhibitor caffeine--inhibiting ATM and ATR amongst other kinases--is explained by inability to activat...

Inactivation of the DnaB helicase leads to the collapse and degradation of the replication fork: a comparison to UV-induced arrest.

Replication forks face a variety of structurally diverse impediments that can prevent them from completing their task. The mechanism by which cells overcome these hurdles is likely to vary depending on the nature of the obstacle and the strand in which the impediment is encountered. Both UV-induced DNA damage and thermosensitive replication proteins have been used in model systems to inhibit DN...

Characterization of the Replication Rate and Intermediates Produced Following Hydroxyurea treatment in Escherichia coli

In order to reproduce, all cells must duplicate their genomes accurately. Active replication forks encounter impediments such as DNA adducts, strand breaks, bound proteins, or secondary DNA structures that impair their ability to duplicate the DNA. UV-irradiation causes DNA damage that arrests replication forks and induces distinct intermediates during the recovery process. However, less is kno...